Publications

A Broad Role for Cysteines in Bovine Antibody Diversity.

Haakenson JK, Deiss TC, Warner GF, Mwangi W, Criscitiello MF, Smider VV.

Immunohorizons. 2019 Oct 16;3(10):478-487

Diversity in the Cow Ultralong CDR H3 Antibody Repertoire.

Haakenson JK, Huang R, Smider VV.

Front Immunol. 2018 Jun 4;9:1262.

The Unusual Genetics and Biochemistry of Bovine Immunoglobulins.

Stanfield RL, Haakenson J, Deiss TC, Criscitiello MF, Wilson IA, Smider VV.

Adv Immunol. 2018;137:135-164.

Immunogenetic factors driving formation of ultralong VH CDR3 in Bos taurus antibodies.

Deiss TC, Vadnais M, Wang F, Chen PL, Torkamani A, Mwangi W, Lefranc MP, Criscitiello MF, Smider VV.

Cell Mol Immunol. 2019 Jan;16(1):53-64.

Rapid elicitation of broadly neutralizing antibodies to HIV by immunization in cows.

Sok D, Le KM, Vadnais M, Saye-Francisco KL, Jardine JG, Torres JL, Berndsen ZT, Kong L, Stanfield R, Ruiz J, Ramos A, Liang CH, Chen PL, Criscitiello MF, Mwangi W, Wilson IA, Ward AB, Smider VV, Burton DR.

Nature. 2017 Aug 3;548(7665):108-111.

SMI-Ribosome inactivating protein conjugates selectively inhibit tumor cell growth.

Roy S, Axup JY, Forsyth JS, Goswami RK, Hutchins BM, Bajuri KM, Kazane SA, Smider VV, Felding BH, Sinha SC.

Chem Commun (Camb). 2017 Apr 11;53(30):4234-4237.

Distinct antibody species: structural differences creating therapeutic opportunities.

Muyldermans S, Smider VV.

Curr Opin Immunol. 2016 Jun;40:7-13.

Antibody microarrays utilizing site-specific antibody-oligonucleotide conjugates.

Wold ED, McBride R, Axup JY, Kazane SA, Smider VV.

Bioconjug Chem. 2015 May 20;26(5):807-11.

Unnatural amino acids in novel antibody conjugates.

Hallam TJ, Smider VV.

Future Med Chem. 2014 Jul;6(11):1309-24.

A genetically encoded aza-Michael acceptor for covalent cross-linking of protein-receptor complexes.

Furman JL, Kang M, Choi S, Cao Y, Wold ED, Sun SB, Smider VV, Schultz PG, Kim CH.

J Am Chem Soc. 2014 Jun 11;136(23):8411-7.

Recruiting cytotoxic T cells to folate-receptor-positive cancer cells.

Kularatne SA, Deshmukh V, Gymnopoulos M, Biroc SL, Xia J, Srinagesh S, Sun Y, Zou N, Shimazu M, Pinkstaff J, Ensari S, Knudsen N, Manibusan A, Axup J, Kim C, Smider V, Javahishvili T, Schultz PG.

Angew Chem Int Ed Engl. 2013 Nov 11;52(46):12101-12104.

A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union.

Grover RK, Zhu X, Nieusma T, Jones T, Boreo I, MacLeod AS, Mark A, Niessen S, Kim HJ, Kong L, Assad-Garcia N, Kwon K, Chesi M, Smider VV, Salomon DR, Jelinek DF, Kyle RA, Pyles RB, Glass JI, Ward AB, Wilson IA, Lerner RA.

Science. 2014 Feb 7;343(6171):656-661.

Bispecific small molecule-antibody conjugate targeting prostate cancer.

Kim CH, Axup JY, Lawson BR, Yun H, Tardif V, Choi SH, Zhou Q, Dubrovska A, Biroc SL, Marsden R, Pinstaff J, Smider VV, Schultz PG.

Proc Natl Acad Sci U S A. 2013 Oct 29;110(44):17796-801.

Functional antibody CDR3 fusion proteins with enhanced pharmacological properties.

Zhang Y, Wang D, de Lichtervelde L, Sun SB, Smider VV, Schultz PG, Wang F.

Angew Chem Int Ed Engl. 2013 Aug 5;52(32):8295-8.

Reshaping antibody diversity.

Wang F, Ekiert DC, Ahmad I, Yu W, Zhang Y, Bazirgan O, Torkamani A, Raudsepp T, Mwangi W, Criscitiello MF, Wilson IA, Schultz PG, Smider VV.

Cell. 2013 Jun 6;153(6):1379-93.

Self-assembled antibody multimers through peptide nucleic acid conjugation.

Kazane SA, Axup JY, Kim CH, Ciobanu M, Wold ED, Barluenga S, Hutchins BA, Schultz PG, Winssinger N, Smider VV.

J Am Chem Soc. 2013 Jan 9;135(1):340-6.

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids.

Axup JY, Bajjuri KM, Ritland M, Hutchins BM, Kim CH, Kazane SA, Halder R, Forsyth JS, Santidrian AF, Stafin K, Lu Y, Tran H, Seller AJ, Biroc SL, Szydlik A, Pinkstaff JK, Tian F, Sinha SC, Felding-Habermann B, Smider VV, Schultz PG.

Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16101-6.

Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair.

Tsai C, Smider V, Hwang BJ, Chu G.

Methods Mol Biol. 2012;920:53-78.

Synthesis of bispecific antibodies using genetically encoded unnatural amino acids.

Kim CH, Axup JY, Dubrovska A, Kazane SA, Hutchins BA, Wold ED, Smider VV, Schultz PG.

J Am Chem Soc. 2012 Jun 20;134(24):9918-21.

Arrayed protein library technology for therapeutic biologic discovery.

Mao H, Graziano J, Smider VV.

Biotechnol Genet Eng Rev. 2012;28:131-45.

Site-specific DNA-antibody conjugates for specific and sensitive immuno-PCR.

Kazane SA, Sok D, Cho EH, Uson ML, Kuhn P, Schultz PG, Smider VV.

Proc Natl Acad Sci U S A. 2012 Mar 6;109(10):3731-6.

Selective formation of covalent protein heterodimers with an unnatural amino acid.

Hutchins BM, Kazane SA, Staflin K, Forsyth JS, Felding-Habermann B, Smider VV, Schultz PG.

Chem Biol. 2011 Mar 25;18(3):299-303.

Site-specific coupling and sterically controlled formation of multimeric antibody fab fragments with unnatural amino acids.

Hutchins BM, Kazane SA, Staflin K, Forsyth JS, Felding-Habermann B, Schultz PG, Smider VV.

J Mol Biol. 2011 Mar 4;406(4):595-603.

Spatially addressed combinatorial protein libraries for recombinant antibody discovery and optimization.

Mao H, Graziano JJ, Chase TM, Bentley CA, Bazirgan OA, Reddy NP, Song BD, Smider VV.

Nat Biotechnol. 2010 Nov;28(11):1195-202.

A human germ line antibody light chain with hydrolytic properties associated with multimerization status.

Sharma V, Heriot W, Trisler K, Smider V.

J Biol Chem. 2009 Nov 27;284(48):33079-87.

Mutagenesis and evolution of sulfated antibodies using an expanded genetic code.

Liu CC, Choe H, Farzan M, Smider VV, Schultz PG.

Biochemistry. 2009 Sep 22;48(37):8891-8. doi: 10.1021/bi9011429.

Evolution of proteins with genetically encoded “chemical warheads”.

Liu CC, Mack AV, Brustad EM, Mills JH, Groff D, Smider VV, Schultz PG.

J Am Chem Soc. 2009 Jul 22;131(28):9616-7.

Mechanistic studies of the immunochemical termination of self-tolerance with unnatural amino acids.

Grünewald J, Hunt GS, Dong L, Niessen F, Wen BG, Tsao ML, Perera R, Kang M, Laffitte BA, Azarian S, Ruf W, Nasoff M, Lerner RA, Schultz PG, Smider VV.

Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4337-42.

Protein evolution with an expanded genetic code.

Liu CC, Mack AV, Tsao ML, Mills JH, Lee HS, Choe H, Farzan M, Schultz PG, Smider VV.

Proc Natl Acad Sci U S A. 2008 Nov 18;105(46):17688-93.

Immunochemical termination of self-tolerance.

Grünewald J, Tsao ML, Perera R, Dong L, Niessen F, Wen BG, Kubitz DM, Smider VV, Ruf W, Nasoff M, Lerner RA, Schultz PG.

Proc Natl Acad Sci U S A. 2008 Aug 12;105(32):11276-80.

A metalloantibody that irreversibly binds a protein antigen.

Trisler K, Looger LL, Sharma V, Baker M, Benson DE, Trauger S, Schultz PG, Smider VV.

J Biol Chem. 2007 Sep 7;282(36):26344-53.

Co-expression of antibody fab heavy and light chain genes from separate evolved compatible replicons in E. coli.

Leonard B, Sharma V, Smider V.